Integrating self-endophytic bio-Inoculant to enhance Roselle productivity under reduced chemical fertilization in Aswan, Egypt

Ibrahim, Zohor Ahmed; Soliman, Wagdi S; Konsowa, Osama; Abbas, Mohammed Tawfik

doi:10.21608/ajabs.2025.404381.1014

Integrating self-endophytic bio-Inoculant to enhance Roselle productivity under reduced chemical fertilization in Aswan, Egypt

Document Type : Research article

Authors

¹ Department of Agricultural Microbiology, Faculty of Agriculture and Natural Resources, Aswan University

² Horticulture Department, Faculty of Agriculture and Natural Resources, Aswan University, Aswan 81528, Egypt

³ Department of Biochemistry, Faculty of Agriculture, Cairo University.

10.21608/ajabs.2025.404381.1014

Abstract

Roselle (Hibiscus sabdariffa L.) is a valuable medicinal and economic crop widely cultivated in Egypt, with Aswan contributing 53% of the total area. On the other hand, sustainable agriculture increasingly relies on plant-microbe interactions, particularly endophytic microorganisms. This study investigated the potential of self-endophytic microbiomes as a bioformulation to reduce dependence on chemical fertilizers while enhancing roselle growth and productivity. A field experiment was conducted using different NPK fertilizer levels combined with a mixed self-endophytic inoculum. Results showed that replacing 25% of NPK with bio-inoculation significantly increased fruit number and shoot biomass by 25% and 15%, respectively. Also, using 75% NPK plus bio-inoculation improved seed weight and dry calyx yield by 35% and 16%, respectively, compared to full NPK. Additionally, this treatment enhanced chlorophyll a and b content by 15% and 23%. The highest carotenoid content was observed with 25% NPK plus bio-inoculant with 21% increase. These findings confirm that endophytic bacteria can colonize roselle tissues without causing harm, improve nutrient uptake, and boost productivity. This eco-friendly approach represents a promising strategy for sustainable roselle cultivation, reducing chemical input while maintaining high yields and supporting environmental health.

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